Formation of Mercapturic Acids

نویسندگان

  • H. G. BRAY
  • J. C. CAYGILL
  • SYBIL P. JAMES
  • P. B. WOOD
چکیده

& Young (1959, 1960) showed that n-propylmercapturic acid was excreted by rats, rabbits, guinea pigs and mice to which 1-bromoor 1-iodopropane had been administered. Bray & James (1958) obtained evidence suggesting that 1-bromobutane, -pentane, -hexane, -heptane and -octane, and 1-nitro-ethane and -propane, formed mercapturic acids in the rabbit, and Barnes, James & Wood (1959) showed that a similar reaction occurred with 1-nitrobutane in the rabbit and rat. The evidence was the increased concentration of thiol compounds (as measured by the intensity of the colour given with sodium nitroprusside) observed in the alkali-hydrolysed urines from animals dosed with the bromoalkanes and the chromatographic detection of the mercapturic acids. It was recognized that the nitroprusside reaction was not specific for mercapturic acids and fractionation of the 'apparent mercapturic acid' into ether-soluble (mercapturic acid) and etherinsoluble (sulphur-containing material that was not mercapturic acid) was reported by Bray & James (1960). In the present paper a specific method for the estimation of the aliphatic mercapturic acids is described, and the isolation of

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تاریخ انتشار 2005